Fluorescence Microscopy

There is a big difference between an ordinary eyepiece of an ordinary microscope unit and that of a fluorescence microscopy unit. Other microscope eyepieces do not emit original light as originated from the light source. With the fluorescence microscopes on the other hand, the light which your eyes can see is the light that comes from the microscope specimen which fluoresced, made possible by its high intensity light. This happens when a light passes through a filter tube which is dichroic and which also contains an excited fluorescence bandpass filter. In the filter process however, a certain kind of wavelength is only allowed to get into the specimen. The others which were unable to pass through are emitted through the emission filter.